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Studies On the Culturing and Parasitism of Plant-Parasitic Nematodes, in Particular Ditylenchus Dipsaci and Aphelenchoides Ritzeiwabosi On Alfalfa Tissues

Studies On the Culturing and Parasitism of Plant-Parasitic Nematodes, in Particular Ditylenchus... STUDIES ON THE CULTURING AND PARASITISM OF PLANT- PARASITIC NEMATODES, IN PARTICULAR DITYLENCHUS DIPSACI AND APHELENCHOIDES RITZEIWABOSI ON ALFALFA TISSUES1) BY L. R. KRUSBERG2) Rothamsted Experimental Station, Harpenden, Herts., England Ditylenchus dipsaci and Aphelenchoides ritzemabosi reproduced rapidly on alfalfa callus tissue grown on a nutrient agar medium containing 2,4-dichlorophenoxyacetic acid (2,4-D). Cultures inoculated with 50 nematodes and kept at 22° C contained 40,000 to 80,000 individuals after two months. Pratylenchus zeae and P. penetrans reproduced well and Tylenchorhynchus capitatus and Hoplolaimus coronatus slowly on callus. Four of these species were maintained alive in such cultures for two years without subcultures. The histopathology of D. dipsaci and A. ritzemabosi on alfalfa seedlings grown in soil, seedlings grown aseptically on nutrient agar medium, and callus tissue grown aseptically on nutrient agar medium plus 2,4-D was examined. D. dipsaci penetrated directly through the epidermis in the bases of cotyledons of alfalfa seedlings in soil and formed cavities in cortical parenchyma within 12 hours of inoculation. Infected stems were noticeably swollen at 36 hours after inoculation. Many parenchyma cells were destroyed and others distorted in galled tissue, particularly in young, actively growing shoots. Only cavities were formed in roots of infected seedlings http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png Nematologica Brill

Studies On the Culturing and Parasitism of Plant-Parasitic Nematodes, in Particular Ditylenchus Dipsaci and Aphelenchoides Ritzeiwabosi On Alfalfa Tissues

Krusberg, L.R.
Nematologica , Volume 6 (3): 20 – Jan 1, 1961
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Publisher
Brill
Copyright
Copyright © Koninklijke Brill NV, Leiden, The Netherlands
ISSN
0028-2596
eISSN
1875-2926
DOI
10.1163/187529261X00027
Publisher site
See Article on Publisher Site

Abstract

STUDIES ON THE CULTURING AND PARASITISM OF PLANT- PARASITIC NEMATODES, IN PARTICULAR DITYLENCHUS DIPSACI AND APHELENCHOIDES RITZEIWABOSI ON ALFALFA TISSUES1) BY L. R. KRUSBERG2) Rothamsted Experimental Station, Harpenden, Herts., England Ditylenchus dipsaci and Aphelenchoides ritzemabosi reproduced rapidly on alfalfa callus tissue grown on a nutrient agar medium containing 2,4-dichlorophenoxyacetic acid (2,4-D). Cultures inoculated with 50 nematodes and kept at 22° C contained 40,000 to 80,000 individuals after two months. Pratylenchus zeae and P. penetrans reproduced well and Tylenchorhynchus capitatus and Hoplolaimus coronatus slowly on callus. Four of these species were maintained alive in such cultures for two years without subcultures. The histopathology of D. dipsaci and A. ritzemabosi on alfalfa seedlings grown in soil, seedlings grown aseptically on nutrient agar medium, and callus tissue grown aseptically on nutrient agar medium plus 2,4-D was examined. D. dipsaci penetrated directly through the epidermis in the bases of cotyledons of alfalfa seedlings in soil and formed cavities in cortical parenchyma within 12 hours of inoculation. Infected stems were noticeably swollen at 36 hours after inoculation. Many parenchyma cells were destroyed and others distorted in galled tissue, particularly in young, actively growing shoots. Only cavities were formed in roots of infected seedlings

Journal

Nematologica – Brill

Published: Jan 1, 1961

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