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A TECHNIQUE FOR SCREENING ANTIBIOTICS AGAINST EELWORMS BY DAPHNE BISHOP Glaxo Laboratories Limited, Stoke Poges, Bucks, England TARJAN and CHEO (1956) described several test methods used in the evaluation of chemicals as nematicides. They included a direct toxicity test, with Panagrellus redivizius as a test organism, in which the nematicidal value of a chemical was estimated by the time required for the chemical to produce complete inactivation of the eelworm. P. redivivus is not a plant parasitic nematode, moreover their's was test in vitro and could take no account of any effect the ohemical might have on the plant invasion, development and reproduction of the eel- worm. The following technique has been designed to test the nemati- cidal value of antibiotics against the rootknot eelworm, Meloidogyne incogn.itcz var. acrita Chitwood. In it the effectiveness of the antibiotic was measured by observing first whether the treated eelworms were still able to penetrate the roots and secondly whether, if penetration occurred, there was normal development of the eelworm with the pro- duction of viable eggs from which larvae also capable of penetrating the roots emerged. METHODS . Application of antibiotic to the eelworrns The antibiotic broth was applied to suspensions
Nematologica – Brill
Published: Jan 1, 1958
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