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Changes in spermine levels are involved in the effects of phorbol ester (12-O-tetradecanoylphorbol-13-acetate) and dibutyryl cAMP on the growth of Trypanosoma cruzi

Changes in spermine levels are involved in the effects of phorbol ester... Biogenic Amines , Vol. 17, No. 1, pp. 31– 41 (2002) Ó VSP 2002. Changes in spermine levels are involved in the effects of phorbol ester (12-O-tetradecanoylphorbol-13-acetate) and dibutyryl cAMP on the growth of Trypanosoma cruzi S. HERNÁNDEZ 1 ; 3 , M. SANCHEZ 1 ; 2 and M. SCHWARCZ DE TARLOVSKY 2 ; 3 ; ¤ 1 Departamento de Bioquímica Humana, Facultad de Medicina, 2155 Paraguay 2 Ciclo Basico Comun, Universidad de Buenos Aires, Argentina 3 Universidad Abierta Interamericana (U.A.I.), Buenos Aires, República Argentina Received 12 December 2001; revised 21 January 2002; accepted 15 March 2002 Abstract —The effects of tumour promoter 12-O-tetradecanoylphorbol-13-acetate (TPA), cAMP analogue dibutyryl cAMP (DB) and histamine were studied on arginine decarboxylase activity, polyamine content and growth rate of Trypanosoma cruzi, RA strain epimastigotes. TPA enhanced all these parameters at the early logarithmic phase of parasite grown in two succesive passages through a polyamine free medium. The stimulatory action of TPA was blocked by inhibitors of transcription (actinomycin D), protein synthesis (cycloheximide) and polyamine synthesis ( ® - DL - di uoromethylarginine and cyclohexylamine). Spermine abolished arginine decarboxylase activity and increased the proliferative rate of both control and TPA-treated epimastigotes. In contrast DB http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png Biogenic Amines Brill

Changes in spermine levels are involved in the effects of phorbol ester (12-O-tetradecanoylphorbol-13-acetate) and dibutyryl cAMP on the growth of Trypanosoma cruzi

Hernández; Sanchez; Schwarcz de Tarlovsky
Biogenic Amines , Volume 17 (1): 31 – Jan 1, 2002
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Publisher
Brill
Copyright
© 2002 Koninklijke Brill NV, Leiden, The Netherlands
ISSN
0168-8561
eISSN
1569-3910
DOI
10.1163/15693910260519283
Publisher site
See Article on Publisher Site

Abstract

Biogenic Amines , Vol. 17, No. 1, pp. 31– 41 (2002) Ó VSP 2002. Changes in spermine levels are involved in the effects of phorbol ester (12-O-tetradecanoylphorbol-13-acetate) and dibutyryl cAMP on the growth of Trypanosoma cruzi S. HERNÁNDEZ 1 ; 3 , M. SANCHEZ 1 ; 2 and M. SCHWARCZ DE TARLOVSKY 2 ; 3 ; ¤ 1 Departamento de Bioquímica Humana, Facultad de Medicina, 2155 Paraguay 2 Ciclo Basico Comun, Universidad de Buenos Aires, Argentina 3 Universidad Abierta Interamericana (U.A.I.), Buenos Aires, República Argentina Received 12 December 2001; revised 21 January 2002; accepted 15 March 2002 Abstract —The effects of tumour promoter 12-O-tetradecanoylphorbol-13-acetate (TPA), cAMP analogue dibutyryl cAMP (DB) and histamine were studied on arginine decarboxylase activity, polyamine content and growth rate of Trypanosoma cruzi, RA strain epimastigotes. TPA enhanced all these parameters at the early logarithmic phase of parasite grown in two succesive passages through a polyamine free medium. The stimulatory action of TPA was blocked by inhibitors of transcription (actinomycin D), protein synthesis (cycloheximide) and polyamine synthesis ( ® - DL - di uoromethylarginine and cyclohexylamine). Spermine abolished arginine decarboxylase activity and increased the proliferative rate of both control and TPA-treated epimastigotes. In contrast DB

Journal

Biogenic Amines – Brill

Published: Jan 1, 2002

Keywords: ADC; TRYPANOSOMA CRUZI; DIBUTYRYL CAMP; TPA; POLYAMINE; HISTAMINE

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