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Detection and identification of macromolecule-derived radicals by epr spin trapping

Detection and identification of macromolecule-derived radicals by epr spin trapping EPR spectroscopy in conjunction with spin trapping has been employed extensively and successfully to detect and identify low-molecular-weight reactive radicals in biological systems. In contrast, this technique has been relatively infrequently used to investigate high-molecular-weight species generated as a result of radical-induced damage to biological macromolecules, such as DNA, RNA, proteins and carbohydrates, despite the fact that the destruction or alteration of these materials is known to play a key role in a large number of cellular injuries and diseases. Recent progress in the detection and identification of these macromolecular radicals by EPR spin trapping is reviewed, with particular emphasis on supplementary techniques which often yield valuable information as to the nature and identity of the species detected. http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png Research on Chemical Intermediates Springer Journals

Detection and identification of macromolecule-derived radicals by epr spin trapping

Research on Chemical Intermediates , Volume 19 (7) – Jan 1, 1993

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References (18)

Publisher
Springer Journals
Copyright
Copyright © Springer 1993
ISSN
0922-6168
eISSN
1568-5675
DOI
10.1163/156856793x00307
Publisher site
See Article on Publisher Site

Abstract

EPR spectroscopy in conjunction with spin trapping has been employed extensively and successfully to detect and identify low-molecular-weight reactive radicals in biological systems. In contrast, this technique has been relatively infrequently used to investigate high-molecular-weight species generated as a result of radical-induced damage to biological macromolecules, such as DNA, RNA, proteins and carbohydrates, despite the fact that the destruction or alteration of these materials is known to play a key role in a large number of cellular injuries and diseases. Recent progress in the detection and identification of these macromolecular radicals by EPR spin trapping is reviewed, with particular emphasis on supplementary techniques which often yield valuable information as to the nature and identity of the species detected.

Journal

Research on Chemical IntermediatesSpringer Journals

Published: Jan 1, 1993

Keywords: Adduct; Nitroxide; Spin Trap; Spin Adduct; Methaemoglobin

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