Get 20M+ Full-Text Papers For Less Than $1.50/day. Start a 14-Day Trial for You or Your Team.

Learn More →

Nitric Oxide as a Putative Transmitter in Aplysia: Neural Circuits and Membrane Effects

Nitric Oxide as a Putative Transmitter in Aplysia: Neural Circuits and Membrane Effects NITRIC OXIDE AS A PUTATIVE TRANSMITTER IN APLYSIA: NEURAL CIRCUITS AND MEMBRANE EFFECTS by JON W. JACKLET and MATTHIAS GRUHN (Department of Biological Sciences, State University of New York at Albany, Albany, NY 12222, U.S.A.) ABSTRACT Nitric oxide (NO) is proposed as a neurotransmitter in the mammalian CNS. Histo- chemical staining and physiological studies suggest that it may be used as a transmitter in invertebrates as well. We stained the CNS of Aplysia using NADPH-diaphorase as a marker for nitric oxide synthase (NOS) to identify neurons, fibre tracts, neuropil and synaptic terminals where NO may be produced. We found a few (3-6) densely stained neurons in most central ganglia and 30 or so bilaterally represented neurons in the cerebral ganglion. Axons, as well as cell bodies, were stained. They joined well defined stained fibre tracts in the ganglia and interganglionic connectives. Several conspicuous synaptic glomeruli were stained, including the lateral terminus of the optic tract. Antiserum to myomodulin, a transmitter/modulator, also stained the lateral terminus. Peripheral nerves including the optic, tentacular and lip nerves contained stained fibres. Select cerebral E-cluster neurons, impaled with micropipettes, responded to the NO generating compounds SIN-1 (3 morpholino-sydnonimine) and s-nitrosocysteine (S- NC). The http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png Netherlands Journal of Zoology (in 2003 continued as Animal Biology) Brill

Nitric Oxide as a Putative Transmitter in Aplysia: Neural Circuits and Membrane Effects

Download PDF
11 pages

Loading next page...
 
/lp/brill/nitric-oxide-as-a-putative-transmitter-in-aplysia-neural-circuits-and-u5AOMOTj1F

References

References for this paper are not available at this time. We will be adding them shortly, thank you for your patience.

Publisher
Brill
Copyright
© 1993 Koninklijke Brill NV, Leiden, The Netherlands
ISSN
0028-2960
eISSN
1568-542X
DOI
10.1163/156854293X00593
Publisher site
See Article on Publisher Site

Abstract

NITRIC OXIDE AS A PUTATIVE TRANSMITTER IN APLYSIA: NEURAL CIRCUITS AND MEMBRANE EFFECTS by JON W. JACKLET and MATTHIAS GRUHN (Department of Biological Sciences, State University of New York at Albany, Albany, NY 12222, U.S.A.) ABSTRACT Nitric oxide (NO) is proposed as a neurotransmitter in the mammalian CNS. Histo- chemical staining and physiological studies suggest that it may be used as a transmitter in invertebrates as well. We stained the CNS of Aplysia using NADPH-diaphorase as a marker for nitric oxide synthase (NOS) to identify neurons, fibre tracts, neuropil and synaptic terminals where NO may be produced. We found a few (3-6) densely stained neurons in most central ganglia and 30 or so bilaterally represented neurons in the cerebral ganglion. Axons, as well as cell bodies, were stained. They joined well defined stained fibre tracts in the ganglia and interganglionic connectives. Several conspicuous synaptic glomeruli were stained, including the lateral terminus of the optic tract. Antiserum to myomodulin, a transmitter/modulator, also stained the lateral terminus. Peripheral nerves including the optic, tentacular and lip nerves contained stained fibres. Select cerebral E-cluster neurons, impaled with micropipettes, responded to the NO generating compounds SIN-1 (3 morpholino-sydnonimine) and s-nitrosocysteine (S- NC). The

Journal

Netherlands Journal of Zoology (in 2003 continued as Animal Biology) – Brill

Published: Jan 1, 1993

Keywords: membrane resistance; nitric oxide; transmitter; molluscs; NADPH diaphorase; methylene blue; synapses; Aplysia; myomodulin

There are no references for this article.