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Target Cell Selection and Specific Synapse Formation By Identified Ly'Mnaea Neurons in Vitro

Target Cell Selection and Specific Synapse Formation By Identified Ly'Mnaea Neurons in Vitro TARGET CELL SELECTION AND SPECIFIC SYNAPSE FOR- MATION BY IDENTIFIED LY'MNAEA NEURONS IN VITRO by NAWEED I. SYED and GAYNOR E. SPENCER (Departments of Anatomy and Physiology, Respiratory and Neuroscience Research Groups, Faculty of Medicine, HSC 3330-Hospital Drive, N.W, Galgary, Alberta, T2N 4N1, Canada) SUMMARY Our research is aimed at determining both cellular and molecular mechanisms that underlie target cell selection and specific synapse formation in the nervous system. We have developed an in vitro experimental system which utilizes identified pre and post- synaptic neurons from the mollusc Lymnaea stagnalis. A giant presynaptic neuron, the right pedal dorsal 1 (RPeD1), re-establishes its specific synapses in culture with only postsynaptic target cells (such as visceral J, I), but not with non target cells (VF, RPB). In an attempt to begin to elucidate mechanisms that determine the specificity of synap- togenesis between Lymnaea neurons in vitro, we studied the behaviour of both target and non-target cell growth cones as they encountered RPeD1's growth cones for the first time. Utilizing time-lapse video imaging techniques, we demonstrate that RPeD 1 growth cones attract and repel target and non-target cell growth cones respectively. We suggest that the specificity of synaptogenesis between RPeD 1 http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png Netherlands Journal of Zoology (in 2003 continued as Animal Biology) Brill

Target Cell Selection and Specific Synapse Formation By Identified Ly'Mnaea Neurons in Vitro

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Publisher
Brill
Copyright
© 1993 Koninklijke Brill NV, Leiden, The Netherlands
ISSN
0028-2960
eISSN
1568-542X
DOI
10.1163/156854293X00430
Publisher site
See Article on Publisher Site

Abstract

TARGET CELL SELECTION AND SPECIFIC SYNAPSE FOR- MATION BY IDENTIFIED LY'MNAEA NEURONS IN VITRO by NAWEED I. SYED and GAYNOR E. SPENCER (Departments of Anatomy and Physiology, Respiratory and Neuroscience Research Groups, Faculty of Medicine, HSC 3330-Hospital Drive, N.W, Galgary, Alberta, T2N 4N1, Canada) SUMMARY Our research is aimed at determining both cellular and molecular mechanisms that underlie target cell selection and specific synapse formation in the nervous system. We have developed an in vitro experimental system which utilizes identified pre and post- synaptic neurons from the mollusc Lymnaea stagnalis. A giant presynaptic neuron, the right pedal dorsal 1 (RPeD1), re-establishes its specific synapses in culture with only postsynaptic target cells (such as visceral J, I), but not with non target cells (VF, RPB). In an attempt to begin to elucidate mechanisms that determine the specificity of synap- togenesis between Lymnaea neurons in vitro, we studied the behaviour of both target and non-target cell growth cones as they encountered RPeD1's growth cones for the first time. Utilizing time-lapse video imaging techniques, we demonstrate that RPeD 1 growth cones attract and repel target and non-target cell growth cones respectively. We suggest that the specificity of synaptogenesis between RPeD 1

Journal

Netherlands Journal of Zoology (in 2003 continued as Animal Biology)Brill

Published: Jan 1, 1993

Keywords: synapse formation; neurite outgrowth; dopamine; target selection; growth cones; identified neurons; Lymnaea; in vitro

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