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A method for separating juveniles from other stages of the pine wood nematode, Bursaphelenchus xylophilus

A method for separating juveniles from other stages of the pine wood nematode, Bursaphelenchus... Pine wilt disease is a very complex disease. Although it has been studied for more than half a century, the mechanisms of the disease have not been clearly determined (Zhao, 2008). It has been thought that the pine wood nematode (PWN), Bursaphelenchus xylophilus , was the only pathogen causing the disease (Mamiya, 1983; Yang, 2002). However, more recently it has been shown that bacteria associated with the PWN may play a role in the disease pathogenicity (Oku et al ., 1979; Kawazu & Kaneko, 1997; Han et al ., 2003; Zhao et al ., 2003) and that certain bacteria may be symbiotically associated with the PWN (Zhao & Lin, 2005; Kwon et al. , 2010). Further research on the interactions between the axenic PWNs and their associated bacteria will provide new knowledge about the etiology of the disease. To do such research on pine wilt disease large numbers of axenic nematodes are needed. However, few easy and effective methods exist for obtaining axenic PWNs. Axenic PWNs are often obtained by sterilising PWN eggs (Iwahori & Futai, 1985; Zhao & Lin, 2005; Zhu et al ., 2011), because it is difficult to surface-sterilise the nematodes effectively using antibiotics or http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png Nematology Brill

A method for separating juveniles from other stages of the pine wood nematode, Bursaphelenchus xylophilus

Ye, Jianling; Zhang, Jianping; Tao, Jian; Zhao, Boguang
Nematology , Volume 14 (4): 509 – Jan 1, 2012
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References (16)

Publisher
Brill
Copyright
© Koninklijke Brill NV, Leiden, The Netherlands
Subject
Short communication
ISSN
1388-5545
eISSN
1568-5411
DOI
10.1163/156854112X632664
Publisher site
See Article on Publisher Site

Abstract

Pine wilt disease is a very complex disease. Although it has been studied for more than half a century, the mechanisms of the disease have not been clearly determined (Zhao, 2008). It has been thought that the pine wood nematode (PWN), Bursaphelenchus xylophilus , was the only pathogen causing the disease (Mamiya, 1983; Yang, 2002). However, more recently it has been shown that bacteria associated with the PWN may play a role in the disease pathogenicity (Oku et al ., 1979; Kawazu & Kaneko, 1997; Han et al ., 2003; Zhao et al ., 2003) and that certain bacteria may be symbiotically associated with the PWN (Zhao & Lin, 2005; Kwon et al. , 2010). Further research on the interactions between the axenic PWNs and their associated bacteria will provide new knowledge about the etiology of the disease. To do such research on pine wilt disease large numbers of axenic nematodes are needed. However, few easy and effective methods exist for obtaining axenic PWNs. Axenic PWNs are often obtained by sterilising PWN eggs (Iwahori & Futai, 1985; Zhao & Lin, 2005; Zhu et al ., 2011), because it is difficult to surface-sterilise the nematodes effectively using antibiotics or

Journal

Nematology – Brill

Published: Jan 1, 2012

Keywords: axenic; hydrogen peroxide; NaCMC; pine wilt disease; surface-sterilised nematodes

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