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Effect of Pratylenchus zeae infection on the expression of isozyme activities in resistant and susceptible sugarcane clones

Effect of Pratylenchus zeae infection on the expression of isozyme activities in resistant and... AbstractThe constitutive levels of both phenylalanine ammonia-lyase (PAL) andtyrosine ammonia-lyase (TAL) activities in roots and leaves were found to begreater in sugarcane clones resistant to the lesion nematode Pratylenchuszeae than in susceptible clones. Investigations on the sequentialdevelopment of PAL and TAL activities were undertaken in resistant (Co 7717)and susceptible (CoC 671) sugarcane clones infected with P. zeae. PALactivity in both roots and leaves of the resistant clone showed a maximumpercentage increase, compared with non-infected controls, at 35 and 14 daysafter infection (DAI), respectively. However, in the susceptible clone themaximum percentage decrease compared with the non-infected clone wasobserved at 3 and 14 DAI. TAL activity in the resistant clone showed themaximum percentage increase compared with the non-infected control at 28 DAIin roots and 7 DAI in leaves, whereas in the susceptible clone the maximumpercentage decrease compared with the non-infected control was at 35 DAI inroots and 28 DAI in leaves. In experiments on the induction of enzymeresponse to infection by P. zeae, eight resistant and eight susceptibleclones were analysed 14 days after P. zeae infection. Among the eightresistant clones six showed increased PAL activity compared withnon-infected clones. Four susceptible infected clones showed a decrease ascompared with non-infected clones, while the remaining four infected clonesshowed an increased enzyme activity compared to non-infected clones.Superoxide dismutase (SOD) activity increased quantitatively in both rootsand leaves from nematode-infected susceptible clones compared tonon-infected clones. The SOD activity decreased in roots and leaves fromnematode infected resistant clones, when compared to thenon-infected-resistant clones. In native PAGE analysis of SOD and esteraseactivities the major differences between infected and non-infected clones ofresistant and susceptible sugarcane were observed in low and mediumelectrophoretic mobility ranges. http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png Nematology Brill

Effect of Pratylenchus zeae infection on the expression of isozyme activities in resistant and susceptible sugarcane clones

Kathiresan, Thandavarayan; Mehta, Usha K.
Nematology , Volume 7 (5): 12 – Jan 1, 2005
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Publisher
Brill
Copyright
Copyright © Koninklijke Brill NV, Leiden, The Netherlands
ISSN
1388-5545
eISSN
1568-5411
DOI
10.1163/156854105775143035
Publisher site
See Article on Publisher Site

Abstract

AbstractThe constitutive levels of both phenylalanine ammonia-lyase (PAL) andtyrosine ammonia-lyase (TAL) activities in roots and leaves were found to begreater in sugarcane clones resistant to the lesion nematode Pratylenchuszeae than in susceptible clones. Investigations on the sequentialdevelopment of PAL and TAL activities were undertaken in resistant (Co 7717)and susceptible (CoC 671) sugarcane clones infected with P. zeae. PALactivity in both roots and leaves of the resistant clone showed a maximumpercentage increase, compared with non-infected controls, at 35 and 14 daysafter infection (DAI), respectively. However, in the susceptible clone themaximum percentage decrease compared with the non-infected clone wasobserved at 3 and 14 DAI. TAL activity in the resistant clone showed themaximum percentage increase compared with the non-infected control at 28 DAIin roots and 7 DAI in leaves, whereas in the susceptible clone the maximumpercentage decrease compared with the non-infected control was at 35 DAI inroots and 28 DAI in leaves. In experiments on the induction of enzymeresponse to infection by P. zeae, eight resistant and eight susceptibleclones were analysed 14 days after P. zeae infection. Among the eightresistant clones six showed increased PAL activity compared withnon-infected clones. Four susceptible infected clones showed a decrease ascompared with non-infected clones, while the remaining four infected clonesshowed an increased enzyme activity compared to non-infected clones.Superoxide dismutase (SOD) activity increased quantitatively in both rootsand leaves from nematode-infected susceptible clones compared tonon-infected clones. The SOD activity decreased in roots and leaves fromnematode infected resistant clones, when compared to thenon-infected-resistant clones. In native PAGE analysis of SOD and esteraseactivities the major differences between infected and non-infected clones ofresistant and susceptible sugarcane were observed in low and mediumelectrophoretic mobility ranges.

Journal

Nematology – Brill

Published: Jan 1, 2005

Keywords: NATIVE PAGE; TYROSINE AMMONIA-LYASE; SACCHARUM OFFICINARUM; ESTERASE; PHENYLALANINE AMMONIA-LYASE; SUPEROXIDE DISMUTASE; LESION NEMATODE

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