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AbstractSteinernema silvaticum sp. n. is described from woodland soil near Berlin,Germany. The species is quite common in European woodlands, being found inforest soil all over Germany, in Belgium, the Czech Republic, TheNetherlands, Sweden and the UK. It was previously reported as Steinernemasp. 'B' in Germany and the Czech Republic, and as Steinernema sp. 'B3' inUK, Belgian and Dutch surveys. Third-stage infective juveniles are mainlycharacterised by a straight body of medium length (means = 700–900 μm),lateral fields with eight equal ridges (with appearance of nine parallel,equally spaced lines under light microscope), rather broad, flatly roundedand continuous cephalic region, excretory pore at level of mid-pharynx andaverage hyaline tail portion constituting ca half total tail length; maleswith mucronate tail, yellowish spicules of ca 50 μm length and widemanubria; females with short conoid tail with pointed non-mucronate tip.According to the ITS rDNA sequences, S. silvaticum sp. n. belongs to the'feltiae-kraussei-oregonense' group of Steinernema species, although it isclosest to S. kraussei. The new species shows a high level of nucleotidedifferences in ITS rDNA sequences from other steinernematid species and canbe easily distinguished from S. kraussei, S. feltiae, S. oregonense, S.weiseri and S. jollieti by morphological characters of the infectivejuveniles and males.
Nematology – Brill
Published: Jan 1, 2005
Keywords: MORPHOMETRICS; DISTRIBUTION; MOLECULAR DIFFERENTIATION; DESCRIPTION; WOODLAND; GERMANY; ECOLOGY; MORPHOLOGY
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