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AbstractPine wood wilt disease caused by the pine wood nematode, Bursaphelenchusxylophilus , has been a serious problem in the southern regions of Korea.Efficient diagnosis of B. xylophilus from infected pine wood specimens iscritical for the management of this pest. Traditional microscopicexamination often results in an erroneous identification because a closelyrelated non-pathogenic species, B. mucronatus, has a great degree ofmorphological similarity to B. xylophilus. In an attempt to search forreliable molecular markers for the discrimination of these species, we havecloned the 5S rRNA genomic DNA fragments containing both coding andintergenic spacer (IGS) regions from B. xylophilus and B. mucronatus througha homology-probing PCR strategy. Sequence analyses revealed that codingsequences of the 5S rRNA gene from the two species are almost identical(98.3% homology) but that the IGS sequences differ substantially between thespecies. Based on the IGS sequence differences (69.7% homology), we designedspecies-specific primer sets and developed a PCR-based diagnosis protocolfor the identification and discrimination of the two nematode species on amolecular basis.
Nematology – Brill
Published: Jan 1, 2004
Keywords: PINE WOOD WILT DISEASE; SPECIES-SPECIFIC; PINE WOOD NEMATODE; BURSAPHELECHUS MUCRONATUS; DIAGNOSTICS; IGS; 5S RRNA; PCR
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