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Purification of Cyclops Cultures By Ph Shock (Copepoda)

Purification of Cyclops Cultures By Ph Shock (Copepoda) PURIFICATION OF CYCLOPS CULTURES BY PH SHOCK (COPEPODA) BY LORETTA BULKOWSKI, WILLIAM F. KRISE and KEITH A. KRAUS U.S. Fish and Wildlife Service, National Fishery Research and Development Laboratory, R.D. #4, Box 63, Wellsboro, Pennsylvania 16901, U.S.A. INTRODUCTION Cyclops and Daphnia frequently inhabit the same bodies of water and are ex- cellent foods for zooplanktivorous fish. Intensive rearing of Cyclops for research or as a forage species requires the removal of Daphnia to alleviate interspecific competition. Mechanical separation is not completely successful because the screens that are commonly used allow small Daphnia to pass with Cyclops. The objective of this work was to develop a physiological separation technique bas- ed on relative survival of both Daphnia and Cyclops in solutions adjusted to various pH's. Daphnia has repeatedly been shown to be sensitive to low pH. Acid toxicity tests on Daphnia demonstrated that a several-day exposure to pH 4.5 was lethal (Allen, 1980); the organism reportedly does not occur in lakes with a pH below 5 (Confer et al., 1983), and reproduction is unlikely below pH 5.5 (Parent & Cheetham, 1980); among Daphnia exposed to pH 3. 7, 33 % died in 1 hour and 80% in 3 hours http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png Crustaceana Brill

Purification of Cyclops Cultures By Ph Shock (Copepoda)

Crustaceana , Volume 48 (1-3): 179 – Jan 1, 1985

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References (3)

Publisher
Brill
Copyright
© 1985 Koninklijke Brill NV, Leiden, The Netherlands
ISSN
0011-216x
eISSN
1568-5403
DOI
10.1163/156854085X00864
Publisher site
See Article on Publisher Site

Abstract

PURIFICATION OF CYCLOPS CULTURES BY PH SHOCK (COPEPODA) BY LORETTA BULKOWSKI, WILLIAM F. KRISE and KEITH A. KRAUS U.S. Fish and Wildlife Service, National Fishery Research and Development Laboratory, R.D. #4, Box 63, Wellsboro, Pennsylvania 16901, U.S.A. INTRODUCTION Cyclops and Daphnia frequently inhabit the same bodies of water and are ex- cellent foods for zooplanktivorous fish. Intensive rearing of Cyclops for research or as a forage species requires the removal of Daphnia to alleviate interspecific competition. Mechanical separation is not completely successful because the screens that are commonly used allow small Daphnia to pass with Cyclops. The objective of this work was to develop a physiological separation technique bas- ed on relative survival of both Daphnia and Cyclops in solutions adjusted to various pH's. Daphnia has repeatedly been shown to be sensitive to low pH. Acid toxicity tests on Daphnia demonstrated that a several-day exposure to pH 4.5 was lethal (Allen, 1980); the organism reportedly does not occur in lakes with a pH below 5 (Confer et al., 1983), and reproduction is unlikely below pH 5.5 (Parent & Cheetham, 1980); among Daphnia exposed to pH 3. 7, 33 % died in 1 hour and 80% in 3 hours

Journal

CrustaceanaBrill

Published: Jan 1, 1985

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