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NOTES AND NEWS OBSERVATIONS ON THE NARCOTIZATION OF CRUSTACEAN ZOOPLANKTON 1) BY JOHN E. GANNON and SUSAN A. GANNON 2) Center for Great Lakes Studies, University of Wisconsin-Milwaukee, Milwaukee, Wisconsin 53201, U.S.A. INTRODUCTION Crustacean zooplankton that is preserved with formalin undergoes an initial unfavorable reaction to this universal preservative. This reaction, termed here as "formalin frenzy", is expressed as violent locomotor activity prior to death that often causes rejection of gut contents, spilling of eggs from cladoceran brood pouches, and dropping of egg sacs by copepods. Elbourn (1966) suggested that a high percentage of empty guts in Cyclops strenuus stresauus (Fischer) was due to rejection of gut contents when preserved with formalin. We have often noted Daphnia pulex (L.) females to drop eggs from their brood pouches while swimming in an exceedingly erratic manner after the introduction of formalin into the water. These reactions to formalin present problems when samples have been procured expressly for purposes of food habit analyses by direct examination of gut contents or in calculations of secondary productivity involving counts of eggs per female and numbers of females with and without eggs. Consequently, it would be desirable to find a substance that would effectively
Crustaceana – Brill
Published: Jan 1, 1975
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