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ELECTROGRAPHIC STUDIES OF CARDIAC ACTIVITY OF THE CLADOCERAN, DAPHNIA BY J. RENE P. TASSE 1) AND GEORGE CAMOUGIS Department of Biology, Clark University, Worcester, Massachusetts, U.S.A. - INTRODUCTION The heart rate of Daphnia has been reported by several investigators. The in vivo heart rate was observed visually by Pickering (1894), Sollmann & Webb (1941), and Bekker & Krijgsman (1951), and stroboscopically by Baylor (1942). The in situ heart rate was observed visually by Levy (1927). Krijgsman ( 1952 ) quickly pointed out that although these methods are fairly accurate for observing low frequency phenomena, they do not suffice at higher frequencies nor do they detect slight irregularities. Visual observation in most cases was performed at reduced temperatures, and the source of illumination required by both methods of observation may possibly have varied the internal and external factors which influence the heart rate of most organisms. This paper reports the feasibility of recording the heart rate of Daphnia via an electrographic method. Various electrode coupling systems were examined since electrical activity in this cladoceran has never been demonstrated. Secondly, a cursory study on the effects of gamma-aminobutyric acid (GABA) and picrotoxin on the heart rate of Daphnia was made.
Crustaceana – Brill
Published: Jan 1, 1965
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