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Biological Characteristics of the Entomopathogenic Nematode Heterorhabditis Sp. Is-5: a Heat Tolerant Isolate From Israel

Biological Characteristics of the Entomopathogenic Nematode Heterorhabditis Sp. Is-5: a Heat... BIOLOGICAL CHARACTERISTICS OF THE ENTOMOPATHOGENIC NEMATODE HETERORHABDITIS SP. IS-5: A HEAT TOLERANT ISOLATE FROM ISRAEL BY I. GLAZER1), E. KOZODOI1), G. HASHMI2) and R. GAUGLER2) 1)Department of Nematology, ARO, Volcani Centre, Bet Dagan, 50-250, Israel; 2)Department of Entomology, Rutgers University, New Brunswick, NJ, 08903, USA Entomopathogenic nematode isolate (IS-5), from under the canopy of fruit trees in the arid "Negev" region of Israel was found to be highly tolerant to temperatures above 30°C. Morpho- logical measurements did not distinguish this isolate from Heterorhabditis bacteriophora Poinar, 1976. However, DNA extracted from four different genotypes (Heterorhabditis sp. IS-5, HP88 and IS-3 and H. megidis HSH2) amplified with six random primers yielded products from which it was calculated that the similarity between IS-5 and HP88 was 20%, between IS-3 and HP88 85% and between IS-3 and H. megidis 15.6%. Two hours at 37°C killed 79% HP88 infective juveniles but only 18% IS-5. Eight hours at 37°C killed 87% of HP88 but only 28% of IS-5. One hour at 40°C killed all infective juveniles of the HP88 isolate but only 26% of IS-5. Heat shock treatment for 2 h at 37°C before exposure to 40°C enhanced the survival of IS-5 juveniles to http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png Nematologica Brill

Biological Characteristics of the Entomopathogenic Nematode Heterorhabditis Sp. Is-5: a Heat Tolerant Isolate From Israel

Nematologica , Volume 42 (4): 12 – Jan 1, 1996

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Publisher
Brill
Copyright
Copyright © Koninklijke Brill NV, Leiden, The Netherlands
ISSN
0028-2596
eISSN
1875-2926
DOI
10.1163/004525996X00082
Publisher site
See Article on Publisher Site

Abstract

BIOLOGICAL CHARACTERISTICS OF THE ENTOMOPATHOGENIC NEMATODE HETERORHABDITIS SP. IS-5: A HEAT TOLERANT ISOLATE FROM ISRAEL BY I. GLAZER1), E. KOZODOI1), G. HASHMI2) and R. GAUGLER2) 1)Department of Nematology, ARO, Volcani Centre, Bet Dagan, 50-250, Israel; 2)Department of Entomology, Rutgers University, New Brunswick, NJ, 08903, USA Entomopathogenic nematode isolate (IS-5), from under the canopy of fruit trees in the arid "Negev" region of Israel was found to be highly tolerant to temperatures above 30°C. Morpho- logical measurements did not distinguish this isolate from Heterorhabditis bacteriophora Poinar, 1976. However, DNA extracted from four different genotypes (Heterorhabditis sp. IS-5, HP88 and IS-3 and H. megidis HSH2) amplified with six random primers yielded products from which it was calculated that the similarity between IS-5 and HP88 was 20%, between IS-3 and HP88 85% and between IS-3 and H. megidis 15.6%. Two hours at 37°C killed 79% HP88 infective juveniles but only 18% IS-5. Eight hours at 37°C killed 87% of HP88 but only 28% of IS-5. One hour at 40°C killed all infective juveniles of the HP88 isolate but only 26% of IS-5. Heat shock treatment for 2 h at 37°C before exposure to 40°C enhanced the survival of IS-5 juveniles to

Journal

NematologicaBrill

Published: Jan 1, 1996

Keywords: heat tolerance; heat shock; genetic improvement; reproduction

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