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Rflp Analysis in Canary Islands and North European Populations of Potato Cyst Nematodes (Globodera Spp.). I. Analysis of High Copy Fragments

Rflp Analysis in Canary Islands and North European Populations of Potato Cyst Nematodes... RFLP ANALYSIS IN CANARY ISLANDS AND NORTH EUROPEAN POPULATIONS OF POTATO CYST NEMATODES (GLOBODERA SPP.). I. ANALYSIS OF HIGH COPY FRAGMENTS BY J. A. GONZALEZ1), M. S. PHILLIPS2) and D. L. TRUDGILL2) 1) Instituto Universitario de Bio-organica "Antonio Gonzalez" Ctra. La Esperanza, 2, 38206 La Laguna, Tenerife, Spain 2) Department of Zoology, Scottish Crop Research Institute, Invergowrie, Dundee DD2 5DA, Scotland The high copy fragments generated by the digestion of total DNA using four endonucleases (BamHI, EcoRI, EcoRV and HindIII) were analysed in 24 populations of potato cyst nematodes (PCN) from Tenerife (Canary Islands) and compared with nine control populations representing the virulence spectrum found in Europe. Large differences were found between species but no appreciable variation was observed within the nine Canarian and five control populations of Globodera rostochiensis. Within the G. pallida group two populations, the British pathotype Pal population and a Pa3 population from Luffness (Scotland), each had distinct phenotypes whereas the others including the 15 Canarian and four British control populations, could not be distin- guished from the Pa3 control population from Lindley. Keywords: potato cyst nematodes, molecular diagnosis, RFLP analysis. The molecular diagnosis of plant-parasitic nematodes has been reviewed by Burrows (1990) and http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png Nematologica Brill

Rflp Analysis in Canary Islands and North European Populations of Potato Cyst Nematodes (Globodera Spp.). I. Analysis of High Copy Fragments

Gonzalez, J.A.; Phillips, M.S.; Trudgill, D.L.
Nematologica , Volume 41 (1-4): 12 – Jan 1, 1995
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Publisher
Brill
Copyright
Copyright © Koninklijke Brill NV, Leiden, The Netherlands
ISSN
0028-2596
eISSN
1875-2926
DOI
10.1163/003925995X00413
Publisher site
See Article on Publisher Site

Abstract

RFLP ANALYSIS IN CANARY ISLANDS AND NORTH EUROPEAN POPULATIONS OF POTATO CYST NEMATODES (GLOBODERA SPP.). I. ANALYSIS OF HIGH COPY FRAGMENTS BY J. A. GONZALEZ1), M. S. PHILLIPS2) and D. L. TRUDGILL2) 1) Instituto Universitario de Bio-organica "Antonio Gonzalez" Ctra. La Esperanza, 2, 38206 La Laguna, Tenerife, Spain 2) Department of Zoology, Scottish Crop Research Institute, Invergowrie, Dundee DD2 5DA, Scotland The high copy fragments generated by the digestion of total DNA using four endonucleases (BamHI, EcoRI, EcoRV and HindIII) were analysed in 24 populations of potato cyst nematodes (PCN) from Tenerife (Canary Islands) and compared with nine control populations representing the virulence spectrum found in Europe. Large differences were found between species but no appreciable variation was observed within the nine Canarian and five control populations of Globodera rostochiensis. Within the G. pallida group two populations, the British pathotype Pal population and a Pa3 population from Luffness (Scotland), each had distinct phenotypes whereas the others including the 15 Canarian and four British control populations, could not be distin- guished from the Pa3 control population from Lindley. Keywords: potato cyst nematodes, molecular diagnosis, RFLP analysis. The molecular diagnosis of plant-parasitic nematodes has been reviewed by Burrows (1990) and

Journal

Nematologica – Brill

Published: Jan 1, 1995

Keywords: RFLP analysis; potato cyst nematodes; molecular diagnosis

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